pfge method  (Tenova Pharmaceuticals Inc)

Journal: Applied and Environmental Microbiology

Article Title: Rapid and Simple Universal Escherichia coli Genotyping Method Based on Multiple-Locus Variable-Number Tandem-Repeat Analysis Using Single-Tube Multiplex PCR and Standard Gel Electrophoresis

doi: 10.1128/AEM.02812-18

Figure Lengend Snippet: Dendrogram of the MLVA (A) and PFGE (B) results for E. coli O157:H7 strains. The dendrograms were constructed by BioNumerics software using UPGMA (A and B) and the Dice algorithm (A) based on the band profile on the electrophoresis gel. The PFGE profile was initially reported by aNRC-EC at the Robert Debré Hospital. The PFGE types C, C′, S, Sʺ, and Sʺʹ are profiles differentiated by less than three bands and considered similar according to the criteria of Tenover et al. (20). The dotted lines correspond to the 95% similarity cutoff.

Article Snippet: All the 21 O157:H7 E. coli isolates were also typed by the PFGE method by the Associated National Reference Center for E. coli (aNRC-EC) for epidemiological purposes.

Techniques: Construct, Software, Electrophoresis

Journal: The Open Microbiology Journal

Article Title: Trends in the Genetic Background of Methicillin-Resistant Staphylococcus Aureus Clinical Isolates in a South African Hospital: An Institutional-Based Observational Study

doi: 10.2174/1874285801711010339

Figure Lengend Snippet: Frequency of MRSA isolation from clinical specimen and the distribution of the agr groups, the spa clusters, SCC mec types and Pulse field gel electrophoresis clusters among the isolates.

Article Snippet: The PFGE was anlaysed using the method described by Tenova and colleagues [ ], while this method was able to differentiate the clinical S. aureus isolates based on their genetic content, it did not allow the assessment of the genetic relationship between the genotypes.

Techniques: Isolation, Nucleic Acid Electrophoresis, Pulsed-Field Gel, Electrophoresis